Resistance of transgenic papaya trees to papaya ringspot in Brazil

Abstract: The objective of this work was to evaluate the resistance transgenic papaya populations (PTPs) Papaya ringspot virus-P (PRSV-P). 'Sunrise Solo' plants were produced with gene PRSV-P protein coat, and PRSV mechanically inoculated in greenhouse conditions. presence CP/PRSV homozygosis evaluated by polymerase chain reaction (PCR). Selected control transplanted field for agronomic evaluations. conditions showed between 96.3 5.8%, without variation symptoms. PTPs 1/6, 1/7, 1/9, 1/10, 1/15, 2/38, 2/41, 2/56, 2/65, 3/27, 3/46, 3/48, 4/9, 4/27, 8/4, 8/23, 8/33, 18/3, 18/4, 18/8, 18/22, 18/27, 28/97, 28/104, 28/110 no symptoms, they ELISA negative, most them contained CP NPT II genes. 1/6 3/46 had double insertion. 1/6/20, 1/6/59, 1/6/64, 1/6/90, 3/46/44, 3/46/52, 18/27/97 a well-formed fruit cluster, piriform weighing approximately 500 grams, orange pulp, less than 10% carpelloidy. 1/6/59 3/46/52 show PRSV, good characteristics, homozygosis.